intracerebral pathogenicity index meaning in Chinese

脑内致病指数

This strain ' s virulence was judged by mean death time of chick embryos ( mdt ) , intracerebral pathogenicity index in day - old chicks ( icpi ) and intravenous pathogenicity index in 6 - week - old chickens ( ivpi ) and it was found to be the virulent strain . at last , it was tested by the recurrent infection and found that it was the newcastle disease virus ( ndv ) , and it was named hbg - 1 strain . in order to find the difference of the cleavage site of this strain with f48e9 and ? 30 strain , a part of the cleavage site of fusion protein gene fragment was amplified by rt - pcr using a primer and sequenced . the sequence analysis showed this strain had low homology with f4ge9 and cso . a phylogenetic tree based on the published sequences of ndv reference strains was constructed and showed the isolated strain hbg - 1 belonged to the genotype w ndv , a novel genotype ndv
为了进一步探寻分离株与标准株的异同，又采用rt - pcr方法，扩增获得分离株f _ o裂解位点附近的基因片段，经测序后与国际上已发表的新城疫病毒的核酸序列进行比较，结果表明其与标准株和疫苗株之间的同源性较低，仅为82 86之间。经系统发育进化树分析后，判定该分离株为新城疫病毒( ndv )基因型。运用计算机软件对其裂解位点处的氨基酸序列进行预测和分析，结果表明该分离株为新城疫病毒强毒株并具有基因型的典型结构特征。